CD35 Antibody (A4G1-R)

Western Blot: CD35 Antibody (A4G1-R) [NBP3-32150]

Western Blot: CD35 Antibody (A4G1-R) [NBP3-32150] - Western blot analysis of CD35 on different lysates with Mouse anti-CD35 antibody (NBP3-32150) at 1/2,000 dilution.

Lane 1: TF-1 cell lysate
Lane 2: Human kidney tissue lysate
Lane 3: K-562 cell lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 224 kDa
Observed band size: 280 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32150) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CD35 Antibody (A4G1-R) [NBP3-32150]

Immunohistochemistry: CD35 Antibody (A4G1-R) [NBP3-32150] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD35 with Mouse anti-CD35 antibody (NBP3-32150) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32150, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: CD35 Antibody (A4G1-R) [NBP3-32150]

Immunohistochemistry: CD35 Antibody (A4G1-R) [NBP3-32150] - Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Mouse anti-CD35 antibody (NBP3-32150) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32150) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.