CD35 Antibody (To5) [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08279R

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # To5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD35 Antibodies »

Product Specifications

Immunogen

Recombinant human CD35 protein (Uniprot: P17927)

Localization

Cell Surface

Specificity

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

Marker

Follicular Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for CD35 Antibody (To5) [DyLight 550]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (To5) [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD35 Antibody (To5) [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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