CD36 Antibody - BSA Free

Western Blot: CD36 Antibody - BSA Free [NB400-145] -

Western Blot: CD36 Antibody - BSA Free [NB400-145] - CD36 ligand disrupts TLR2-CD36 interaction modulated TLR2 heterodimer-signaling. (A) Confocal imaging of central retina cryosection stained with CD11b (white), CD36 (red), TLR2 (green), & DAPI (blue) from blue light-challenged WT mice. Scale bar = 25 μm. (B) High magnification (3X) shows subretinal CD11b-positive cells (white) with the co-localisation of CD36 (red) & TLR2 (green). Scale bar = 5 μm. (C-F) Peritoneal MPs were stimulated with 300 ng/ml R-FSL1 in the presence of 10−7 M MPE-001 or vehicle. (C) MPE-001 disrupted the interaction between CD36 labeled with Cy5 (red) & TLR2 labeled with Cy3 (green) as assessed by FRET after 5 min stimulation with R-FSL1. (D) Percentage of energy transfer measured using LSM-700 confocal microscope (Zeiss). Data in B,C are representative of 3-4 independent experiments. (E) Phosphorylated & total Western blot density bands of IRAK4, IKK alpha beta & P65-NF kappaB, JNK & P38 in peritoneal MPs from CD36+/+ & CD36−/− mice stimulated with R-FSL1. (F) Quantification of P65-NF kappaB following stimulation of CD36+/+ & CD36−/− peritoneal MPs with R-FSL1 using ELISA-based assay. Data in C-F are representative of 3 independent experiments (n = 3/group). In (D,F) one-way ANOVA test with Newman-Keuls post-test for multiple comparison was performed. *P < 0.05, **P < 0.01 & ***P < 0.001 vs R-FSL1. Data are shown as mean ± S.E.M. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31501473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-145] -

Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-145] - CD36 ligand disrupts TLR2-CD36 interaction modulated TLR2 heterodimer-signaling. (A) Confocal imaging of central retina cryosection stained with CD11b (white), CD36 (red), TLR2 (green), & DAPI (blue) from blue light-challenged WT mice. Scale bar = 25 μm. (B) High magnification (3X) shows subretinal CD11b-positive cells (white) with the co-localisation of CD36 (red) & TLR2 (green). Scale bar = 5 μm. (C-F) Peritoneal MPs were stimulated with 300 ng/ml R-FSL1 in the presence of 10−7 M MPE-001 or vehicle. (C) MPE-001 disrupted the interaction between CD36 labeled with Cy5 (red) & TLR2 labeled with Cy3 (green) as assessed by FRET after 5 min stimulation with R-FSL1. (D) Percentage of energy transfer measured using LSM-700 confocal microscope (Zeiss). Data in B,C are representative of 3-4 independent experiments. (E) Phosphorylated & total Western blot density bands of IRAK4, IKK alpha beta & P65-NF kappaB, JNK & P38 in peritoneal MPs from CD36+/+ & CD36−/− mice stimulated with R-FSL1. (F) Quantification of P65-NF kappaB following stimulation of CD36+/+ & CD36−/− peritoneal MPs with R-FSL1 using ELISA-based assay. Data in C-F are representative of 3 independent experiments (n = 3/group). In (D,F) one-way ANOVA test with Newman-Keuls post-test for multiple comparison was performed. *P < 0.05, **P < 0.01 & ***P < 0.001 vs R-FSL1. Data are shown as mean ± S.E.M. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31501473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-145] -

Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-145] - CD36 ligand disrupts TLR2-CD36 interaction modulated TLR2 heterodimer-signaling. (A) Confocal imaging of central retina cryosection stained with CD11b (white), CD36 (red), TLR2 (green), & DAPI (blue) from blue light-challenged WT mice. Scale bar = 25 μm. (B) High magnification (3X) shows subretinal CD11b-positive cells (white) with the co-localisation of CD36 (red) & TLR2 (green). Scale bar = 5 μm. (C-F) Peritoneal MPs were stimulated with 300 ng/ml R-FSL1 in the presence of 10−7 M MPE-001 or vehicle. (C) MPE-001 disrupted the interaction between CD36 labeled with Cy5 (red) & TLR2 labeled with Cy3 (green) as assessed by FRET after 5 min stimulation with R-FSL1. (D) Percentage of energy transfer measured using LSM-700 confocal microscope (Zeiss). Data in B,C are representative of 3-4 independent experiments. (E) Phosphorylated & total Western blot density bands of IRAK4, IKK alpha beta & P65-NF kappaB, JNK & P38 in peritoneal MPs from CD36+/+ & CD36−/− mice stimulated with R-FSL1. (F) Quantification of P65-NF kappaB following stimulation of CD36+/+ & CD36−/− peritoneal MPs with R-FSL1 using ELISA-based assay. Data in C-F are representative of 3 independent experiments (n = 3/group). In (D,F) one-way ANOVA test with Newman-Keuls post-test for multiple comparison was performed. *P < 0.05, **P < 0.01 & ***P < 0.001 vs R-FSL1. Data are shown as mean ± S.E.M. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31501473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-145] -

Immunocytochemistry/ Immunofluorescence: CD36 Antibody - BSA Free [NB400-145] - CD36 ligand disrupts TLR2-CD36 interaction modulated TLR2 heterodimer-signaling. (A) Confocal imaging of central retina cryosection stained with CD11b (white), CD36 (red), TLR2 (green), & DAPI (blue) from blue light-challenged WT mice. Scale bar = 25 μm. (B) High magnification (3X) shows subretinal CD11b-positive cells (white) with the co-localisation of CD36 (red) & TLR2 (green). Scale bar = 5 μm. (C-F) Peritoneal MPs were stimulated with 300 ng/ml R-FSL1 in the presence of 10−7 M MPE-001 or vehicle. (C) MPE-001 disrupted the interaction between CD36 labeled with Cy5 (red) & TLR2 labeled with Cy3 (green) as assessed by FRET after 5 min stimulation with R-FSL1. (D) Percentage of energy transfer measured using LSM-700 confocal microscope (Zeiss). Data in B,C are representative of 3-4 independent experiments. (E) Phosphorylated & total Western blot density bands of IRAK4, IKK alpha beta & P65-NF kappaB, JNK & P38 in peritoneal MPs from CD36+/+ & CD36−/− mice stimulated with R-FSL1. (F) Quantification of P65-NF kappaB following stimulation of CD36+/+ & CD36−/− peritoneal MPs with R-FSL1 using ELISA-based assay. Data in C-F are representative of 3 independent experiments (n = 3/group). In (D,F) one-way ANOVA test with Newman-Keuls post-test for multiple comparison was performed. *P < 0.05, **P < 0.01 & ***P < 0.001 vs R-FSL1. Data are shown as mean ± S.E.M. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31501473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.