CD39/ENTPD1 Antibody (JA90-36)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67230
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JA90-36
Concentration
1 mg/ml
Product Summary for CD39/ENTPD1 Antibody (JA90-36)
Immunogen
Synthetic peptide within Human CD39/ENTPD1 aa 371-420 / 510. (SwissProt: P49961 Human; SwissProt: P55772 Mouse; SwissProt: P97687 Rat)
Localization
Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD39/ENTPD1 Antibody (JA90-36)
Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]
Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Western blot analysis of CD39/ENTPD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: mouse spleen tissue lysateImmunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD39/ENTPDI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human placenta tissue using anti-CD39 antibody. Counter stained with hematoxylin.Applications for CD39/ENTPD1 Antibody (JA90-36)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD39/ENTPD1
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Alternate Names
ADPase, ATP-diphosphatase, CD39, ENTPD1, NTPD1
Gene Symbol
ENTPD1
Additional CD39/ENTPD1 Products
Product Documents for CD39/ENTPD1 Antibody (JA90-36)
Product Specific Notices for CD39/ENTPD1 Antibody (JA90-36)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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