CD40/TNFRSF5 Antibody (5C3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43416
Clone 5C3 was used by HLDA to establish CD designation.
![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]](https://resources.bio-techne.com/images/products/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0004.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5C3
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD40.
Reactivity Notes
Mouse reactivity reported in scientific literature (Chechneva O et al).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CD40/TNFRSF5 Antibody (5C3) - BSA Free
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the PE conjugate of NBP1-43416. Analysis of human peripheral blood leukocytes using CD40 PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.![Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]](https://resources.bio-techne.com/images/products/CD40-TNFRSF5-Antibody-5C3-Flow-Cytometry-NBP1-43416-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]")
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) - BSA Free [NBP1-43416]
Flow Cytometry: CD40/TNFRSF5 Antibody (5C3) [NBP1-43416] - Analysis using the FITC conjugate of NBP1-43416. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (blue histogram) or Anti-Human CD40 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.Applications for CD40/TNFRSF5 Antibody (5C3) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Application Notes
The 5C3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. This antibody may also be used for immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD40/TNFRSF5
Alternate Names
CD40, TNFRSF5
Gene Symbol
CD40
Additional CD40/TNFRSF5 Products
Product Documents for CD40/TNFRSF5 Antibody (5C3) - BSA Free
Product Specific Notices for CD40/TNFRSF5 Antibody (5C3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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