CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28787

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG2 Clone # selicrelumab

Concentration

LYOPH mg/ml

View all CD40/TNFRSF5 Antibodies »

Product Specifications

Immunogen

TNFRSF5 / CD40

Clonality

Monoclonal

Host

Human

Isotype

IgG2

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Also known as 'selicrelumab'.

IgG2SA is human IgG2 with S378A mutation.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

In vivo assay: CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA[NBP3-28787] -

Compared with the negative control group, selicrelumab had a significant increase in serum ALT content at a dose of 20 mpk, and a trend of increase in serum ALT at a dose of 1 mpk, but there was no statistical difference. AST indexes were all elevated and dose-dependent, but there was no statistical difference.
CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

In vivo assay: CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA[NBP3-28787] -

Compared with the negative control group, selicrelumab had a significant increase in serum ALT content at a dose of 20 mpk, and a trend of increase in serum ALT at a dose of 1 mpk, but there was no statistical difference. AST indexes were all elevated and dose-dependent, but there was no statistical difference.
CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

ELISA: CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA[NBP3-28787] -

Immobilized human CD40 His at 2 ug/mL can bind CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA, EC50=0.009782 ug/mL.
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Applications for CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

UniProt

P25942

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD40/TNFRSF5 Antibody (selicrelumab) - IgG2SA

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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