CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48380
Clone 84-3C1 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted [NBP2-48380]](https://resources.bio-techne.com/images/products/CD43-Sialophorin-Antibody-84-3C1-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48380-img0004.jpg "Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted [NBP2-48380]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 84-3C1
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted [NBP2-48380]
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted [NBP2-48380] - Human Tonsil stained with CD43 Monoclonal Antibody (84-3C1).Applications for CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 1q32.2
Molecular weight of antigen: 90-110kDa
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Molecular weight of antigen: 90-110kDa
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD43
Long Name
Cluster of Differentiation 43
Alternate Names
CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN
Gene Symbol
SPN
Additional CD43 Products
Product Documents for CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted
Product Specific Notices for CD43/Sialophorin Antibody (84-3C1) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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