CD43/Sialophorin Antibody (SPN/1094)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44651
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]](https://resources.bio-techne.com/images/products/CD43-Sialophorin-Antibody-SPN-1094-Immunohistochemistry-Paraffin-NBP2-44651-img0001.jpg "Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]")
![Western Blot: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]](https://resources.bio-techne.com/images/products/CD43-Sialophorin-Antibody-SPN-1094-Western-Blot-NBP2-44651-img0004.jpg "Western Blot: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPN/1094
Concentration
0.2 mg/ml
Product Summary for CD43/Sialophorin Antibody (SPN/1094)
Immunogen
Recombinant full-length human CD43/Sialophorin protein (Uniprot: P16150)
Localization
Cell surface
Specificity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47880)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD43/Sialophorin Antibody (SPN/1094)
Western Blot: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]
Western Blot: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Antibody (SPN/1094).![Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]](https://resources.bio-techne.com/images/products/CD43-Sialophorin-Antibody-SPN-1094-Immunocytochemistry-Immunofluorescence-NBP2-44651-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]")
Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]
Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (SPN/1094) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot®.![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]](https://resources.bio-techne.com/images/products/CD43-Sialophorin-Antibody-SPN-1094-Immunohistochemistry-Paraffin-NBP2-44651-img0002.jpg "Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]")
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651]
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/1094) [NBP2-44651] - Human Tonsil stained with CD43 Monoclonal Antibody (SPN/1094).Applications for CD43/Sialophorin Antibody (SPN/1094)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD43
Long Name
Cluster of Differentiation 43
Alternate Names
CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN
Gene Symbol
SPN
UniProt
Additional CD43 Products
Product Documents for CD43/Sialophorin Antibody (SPN/1094)
Product Specific Notices for CD43/Sialophorin Antibody (SPN/1094)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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