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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Inhibition Activity, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 1M7.8.1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

Low endotoxin

Scientific Data Images for CD44 Antibody (1M7.8.1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (1M7.8.1) - Azide and BSA Free [NBP2-22436]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (1M7.8.1) - Azide and BSA Free [NBP2-22436]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 (green) showing staining in the membrane of BAF-3 cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: CD44 Antibody (1M7.8.1) - Azide and BSA Free [NBP2-22436]

Flow Cytometry: CD44 Antibody (1M7.8.1) - Azide and BSA Free [NBP2-22436]

Flow Cytometry: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 in Hela cells (green) compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (1M7.8.1) - Azide and BSA Free [NBP2-22436]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (1M7.8.1) - Azide and BSA Free [NBP2-22436]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (1M7.8.1) [NBP2-22436] - Analysis of CD44 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left).

Applications for CD44 Antibody (1M7.8.1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

25406 (Rat)

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (1M7.8.1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD44 Antibody (1M7.8.1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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