CD44 Antibody (4B7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26681

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B7

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD44 Antibodies »

Product Specifications

Immunogen

Synthesized peptide [UniProt P16070]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD44 Antibody (4B7) - BSA Free

CD44 Antibody (4B7)

Immunohistochemistry: CD44 Antibody (4B7) [NBP3-26681] -

Immunohistochemistry: CD44 Antibody (4B7) [NBP3-26681] - Image of CD44 Antibody (4B7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
CD44 Antibody (4B7)

Western Blot: CD44 Antibody (4B7) [NBP3-26681] -

Western Blot: CD44 Antibody (4B7) [NBP3-26681] - Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.
All lanes: CD44 Antibody at 1.25ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 82 KDa
Observed band size: 82 KDa

Applications for CD44 Antibody (4B7) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:500

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (4B7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD44 Antibody (4B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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