CD44 Antibody (Hermes-1) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81088CL1
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Goat, Primate, Rabbit, Sheep
Applications
Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # Hermes-1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).
Specificity
This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for CD44 Antibody (Hermes-1) [CoraFluor™ 1]
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (Hermes-1) [CoraFluor™ 1]
Product Specific Notices for CD44 Antibody (Hermes-1) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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