CD44 Antibody (OX-50) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1317
![Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]](https://resources.bio-techne.com/images/products/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0003.jpg "Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-50
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
Localization
Type I membrane protein.
Specificity
This recognizes the rat CD44 cell surface antigen, expressed by T cells, B cells, macrophages and thymocytes.
Marker
Cell Membrane Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD44 Antibody (OX-50) - BSA Free
Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]
Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.![Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]](https://resources.bio-techne.com/images/products/CD44-Antibody-OX-50-Immunohistochemistry-Paraffin-NB600-1317-img0005.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]")
Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]
Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) [NB600-1317] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaining. This antibody clone specifically stained the membranes of the cells close to the bases of the crypts/villi which are known to actively migrate towards the tip of villi.![Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]](https://resources.bio-techne.com/images/products/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0002.jpg "Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]")
Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]
Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Applications for CD44 Antibody (OX-50) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 31229690)
Immunohistochemistry
1:50 - 1:100
Immunohistochemistry-Frozen
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Immunohistochemistry-Paraffin
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Western Blot
reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using NB600-1317 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (OX-50) - BSA Free
Product Specific Notices for CD44 Antibody (OX-50) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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