CD44 Antibody (OX-50) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1317

Novus Biologicals, part of Bio-Techne
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NB600-1317SS
NB600-1317
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-50

Format

BSA Free

Concentration

1.0 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.

Localization

Type I membrane protein.

Specificity

This recognizes the rat CD44 cell surface antigen, expressed by T cells, B cells, macrophages and thymocytes.

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD44 Antibody (OX-50) - BSA Free

Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]

Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]

Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.
Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]

Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) - BSA Free [NB600-1317]

Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) [NB600-1317] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaining. This antibody clone specifically stained the membranes of the cells close to the bases of the crypts/villi which are known to actively migrate towards the tip of villi.
Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]

Flow Cytometry: CD44 Antibody (OX-50) - BSA Free [NB600-1317]

Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for CD44 Antibody (OX-50) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50 - 1:100

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31229690)

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Frozen

1:50 - 1:100. Use reported in scientific literature (PMID 16673425)

Immunohistochemistry-Paraffin

1:50 - 1:100. Use reported in scientific literature (PMID 16673425)

Western Blot

reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 3 using NB600-1317 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a glycoprotrein of 85 kDa, with expression being increased following activation. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (OX-50) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD44 Antibody (OX-50) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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