CD44 Antibody (VFF-17) - variant 7/8

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65535

Novus Biologicals, part of Bio-Techne
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NB100-65535
NB100-65535-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # VFF-17

Concentration

1.0 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Made to Human CD44v7/8

Specificity

Specifically recognizes an epitope encoded by exons v7-v8 on the variant portion of human CD44.

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CD44 Antibody (VFF-17) - variant 7/8

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 - Azide and BSA Free [NB100-65535]

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 - Azide and BSA Free [NB100-65535]

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of representative areas of synovial tissue samples taken from patients with end-stage osteoarthritis (OA) or refractory rheumatoid arthritis (RA) is shown. Expression pattern and staining intensity of RA samples represent the stronger intensity of synovial inflammation when compared with OA samples.
Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 - Azide and BSA Free [NB100-65535]

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 - Azide and BSA Free [NB100-65535]

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of activation markers in synovial membranes of osteoarthritis. Phenotypic markers that are instrumental in extracellular matrix alteration are detectable only at a low level (MMP-1, CD44v7/8) in areas that are slightly FAP-positive. FAP, fibroblast activation protein; MMP, matrix metalloproteinase.
Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 - Azide and BSA Free [NB100-65535]

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 - Azide and BSA Free [NB100-65535]

Immunohistochemistry: CD44 Antibody (VFF-17) - variant 7/8 [NB100-65535] - Analysis of activation markers in synovial membranes of rheumatoid arthritis. Expression of fibroblast activation protein (FAP) is accompanied by accumulation of activation markers (MMP-1 and MMP-13) and splice variants of CD44 (v3 and v7/8) that are known to correlate with the degree of inflammation and that are involved in extracellular matrix deposition.

Applications for CD44 Antibody (VFF-17) - variant 7/8

Application
Recommended Usage

Flow Cytometry

1:10-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (VFF-17) - variant 7/8

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD44 Antibody (VFF-17) - variant 7/8

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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