Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # A20

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all CD45.1 Antibodies »

Product Specifications

Immunogen

SJL mouse thymocytes and splenocytes

Specificity

Mouse CD 45.1 (Leukocyte Common Antigen)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD45.1 Antibody (A20) - Azide and BSA Free

Flow Cytometry: CD45.1 Antibody (A20) - Azide and BSA Free [NBP1-28070]

Flow Cytometry: CD45.1 Antibody (A20) - Azide and BSA Free [NBP1-28070]

Flow Cytometry: CD45.1 Antibody (A20) [NBP1-28070] - SJL mouse splenocytes were stained with Mouse Anti-Mouse CD45.1-UNLB (SB Cat. No. 1795-01) followed by Goat Anti-Mouse IgG2a, Human ads-AF647.

Applications for CD45.1 Antibody (A20) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD45.1

The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. (1, 2) This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. (1, 2) The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variant CD45R, and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. (3, 4) The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.

Long Name

Cluster of Differentiation 45.1

Alternate Names

CD45.1, Ly-5.1

Gene Symbol

PTPRC

Additional CD45.1 Products

Product Documents for CD45.1 Antibody (A20) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD45.1 Antibody (A20) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.