CD45RA Antibody (PTPRC/1131) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54336
![Western Blot: CD45RA Antibody (PTPRC/1131)Azide and BSA Free [NBP2-54336]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-1131-Azide-and-BSA-Free-Western-Blot-NBP2-54336-img0002.jpg "Western Blot: CD45RA Antibody (PTPRC/1131)Azide and BSA Free [NBP2-54336]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PTPRC/1131
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Marker
Leucocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
213 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44845).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RA Antibody (PTPRC/1131) - Azide and BSA Free
Western Blot: CD45RA Antibody (PTPRC/1131)Azide and BSA Free [NBP2-54336]
Western Blot: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1131).![Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-1131-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54336-img0001.jpg "Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336]")
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336]
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1131) - Azide and BSA Free [NBP2-54336] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1131).Applications for CD45RA Antibody (PTPRC/1131) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD45RA
Long Name
CD45R, A isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RA Products
Product Documents for CD45RA Antibody (PTPRC/1131) - Azide and BSA Free
Product Specific Notices for CD45RA Antibody (PTPRC/1131) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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