CD45RA Antibody (PTPRC/1148)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44848
![Western Blot: CD45RA Antibody (PTPRC/1148) [NBP2-44848]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-1148-Western-Blot-NBP2-44848-img0002.jpg "Western Blot: CD45RA Antibody (PTPRC/1148) [NBP2-44848]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PTPRC/1148
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human T-lymphocytes (Uniprot: P08575)
Localization
Cell Surface
Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Marker
Leucocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54335)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RA Antibody (PTPRC/1148)
Western Blot: CD45RA Antibody (PTPRC/1148) [NBP2-44848]
Western Blot: CD45RA Antibody (PTPRC/1148) [NBP2-44848] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/1148).![Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1148) [NBP2-44848]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-1148-Immunohistochemistry-Paraffin-NBP2-44848-img0001.jpg "Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1148) [NBP2-44848]")
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1148) [NBP2-44848]
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/1148) [NBP2-44848] - Human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1148).Applications for CD45RA Antibody (PTPRC/1148)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD45RA
Long Name
CD45R, A isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
UniProt
Additional CD45RA Products
Product Documents for CD45RA Antibody (PTPRC/1148)
Product Specific Notices for CD45RA Antibody (PTPRC/1148)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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