CD45RA Antibody (PTPRC/7018R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13757
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG1 kappa Clone # PTPRC/7018R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human CD45RA protein (Uniprot: P08575)
Localization
Cell surface and cytoplasmic
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14160)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 180-220kDa
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 180-220kDa
Scientific Data Images for CD45RA Antibody (PTPRC/7018R)
CD45RA Antibody (PTPRC/7018R)
Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA antibody (PTPRC/7018R). Inset: PBS instead of primary antibody; secondary only negative control.CD45RA Antibody (PTPRC/7018R)
Formalin-fixed, paraffin-embedded human tonsil stained with CD45RA antibody (PTPRC/7018R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for CD45RA Antibody (PTPRC/7018R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5-1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD45RA
Long Name
CD45R, A isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
UniProt
Additional CD45RA Products
Product Documents for CD45RA Antibody (PTPRC/7018R)
Product Specific Notices for CD45RA Antibody (PTPRC/7018R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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