CD45RA Antibody (PTPRC/818) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47957
![Flow Cytometry: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-818-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47957-img0003.jpg "Flow Cytometry: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]")
![Western Blot: CD45RA Antibody (PTPRC/818)Azide and BSA Free [NBP2-47957]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-818-Azide-and-BSA-Free-Western-Blot-NBP2-47957-img0005.jpg "Western Blot: CD45RA Antibody (PTPRC/818)Azide and BSA Free [NBP2-47957]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # PTPRC/818
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Stimulated human leukocytes
Localization
Cell surface
Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Marker
Leucocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44844).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RA Antibody (PTPRC/818) - Azide and BSA Free
Western Blot: CD45RA Antibody (PTPRC/818)Azide and BSA Free [NBP2-47957]
Western Blot: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957] - Human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (PTPRC/818).![Immunocytochemistry/ Immunofluorescence: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-818-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47957-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]")
Immunocytochemistry/ Immunofluorescence: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]
Immunocytochemistry/Immunofluorescence: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957] - PFA-fixed Jurkat cells labeling CD45RA with CD45RA MAb (PTPRC/818) followed by Goat anti-Mouse IgG CF488 (Green). The nuclear counterstain is Redot.![Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]](https://resources.bio-techne.com/images/products/CD45RA-Antibody-PTPRC-818-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47957-img0001.jpg "Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]")
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957]
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) - Azide and BSA Free [NBP2-47957] - Human tonsil stained with CD45RA Mouse Monoclonal Antibody (PTPRC/818).Applications for CD45RA Antibody (PTPRC/818) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD45RA
Long Name
CD45R, A isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RA Products
Product Documents for CD45RA Antibody (PTPRC/818) - Azide and BSA Free
Product Specific Notices for CD45RA Antibody (PTPRC/818) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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