CD45RB Antibody (BRA-11 (same as BRA-11G))

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33021

Novus Biologicals, part of Bio-Techne
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NBP2-33021-0.1mg
NBP2-33021-0.02mg
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Key Product Details

Species Reactivity

Human, Monkey (Negative), Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BRA-11 (same as BRA-11G)

Concentration

0.2 mg/ml

View all CD45RB Antibodies »

Product Specifications

Immunogen

Non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V)

Reactivity Notes

Does not react with Rat or Rhesus Monkey.

Localization

Cell surface and cytoplasmic

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34564)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RB Antibody (BRA-11 (same as BRA-11G))

Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021]

Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021]

Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021] - Western Blot Analysis of human Raji cell lysate using CD45RB Antibody (BRA-11 (same as BRA-11G)).
Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021]

Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021]

Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021] - Formalin-fixed, paraffin-embedded human tonsil stained with CD45RB Ab (BRA-11).
Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021]

Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021]

Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) [NBP2-33021] - Flow Cytometric Analysis of PFA-fixed Raji cells using CD45RB Antibody (BRA-11 (same as BRA-11G)).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).

Applications for CD45RB Antibody (BRA-11 (same as BRA-11G))

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD45RB

CD45RB is an alternatively spliced form of CD45, a cell surface glycoprotein. It is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases.

Long Name

CD45R, B isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

P08575

Additional CD45RB Products

Product Documents for CD45RB Antibody (BRA-11 (same as BRA-11G))

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD45RB Antibody (BRA-11 (same as BRA-11G))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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