CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08924

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PTPRC/2877R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD45RB Antibodies »

Product Specifications

Immunogen

Recombinant human full-length CD45RB protein (Uniprot: P08575)

Reactivity Notes

Does not react with Rat or Rhesus Monkey.

Localization

Cell Surface

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07180).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

Western Blot: CD45RB Antibody (PTPRC/2877R)Azide and BSA Free [NBP3-08924]

Western Blot: CD45RB Antibody (PTPRC/2877R)Azide and BSA Free [NBP3-08924]

Western Blot: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Western Blot Analysis of human spleen cell lysate using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).
Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924]

Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924]

Immunohistochemistry-Paraffin: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Formalin-fixed, paraffin-embedded human Lymph Node stained with CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R).
Flow Cytometry: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924]

Flow Cytometry: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924]

Flow Cytometry: CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free [NBP3-08924] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using CD45RB Rabbit Recombinant Monoclonal Antibody (PTPRC/2877R) followed by Goat anti-rabbitIgG-CF488 (Blue); Isotype Control (Red).
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Applications for CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD45RB

CD45RB is an alternatively spliced form of CD45, a cell surface glycoprotein. It is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases.

Long Name

CD45R, B isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

Additional CD45RB Products

Product Documents for CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD45RB Antibody (PTPRC/2877R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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