CD45RO Antibody (SPM125) [mFluor Violet 450 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34735MFV450
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Chimpanzee, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM125
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Reactivity Notes
Common Marmoset
Localization
Cell surface
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for CD45RO Antibody (SPM125) [mFluor Violet 450 SE]
CD45RO Antibody (SPM125) [mFluor Violet 450 SE] [NBP2-34735MFV450] -
CD45RO Antibody (SPM125) [mFluor Violet 450 SE] [NBP2-34735MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.Applications for CD45RO Antibody (SPM125) [mFluor Violet 450 SE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CD45RO
Long Name
CD45R, O isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RO Products
Product Documents for CD45RO Antibody (SPM125) [mFluor Violet 450 SE]
Product Specific Notices for CD45RO Antibody (SPM125) [mFluor Violet 450 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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