CD45RO Antibody (T200/797)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44860
![Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) [NBP2-44860]](https://resources.bio-techne.com/images/products/CD45RO-Antibody-T200-797-Immunohistochemistry-Paraffin-NBP2-44860-img0001.jpg "Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) [NBP2-44860]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # T200/797
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human T-lymphocytes (Uniprot: P08575)
Localization
Cell surface
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54315)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RO Antibody (T200/797)
Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) [NBP2-44860]
Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) [NBP2-44860] - Human Tonsil stained with CD45RO Monoclonal Antibody (T200/797).![Flow Cytometry: CD45RO Antibody (T200/797) [NBP2-44860]](https://resources.bio-techne.com/images/products/CD45RO-Antibody-T200-797-Flow-Cytometry-NBP2-44860-img0003.jpg "Flow Cytometry: CD45RO Antibody (T200/797) [NBP2-44860]")
Flow Cytometry: CD45RO Antibody (T200/797) [NBP2-44860]
Flow Cytometry: CD45RO Antibody (T200/797) [NBP2-44860] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (T200/797) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).Applications for CD45RO Antibody (T200/797)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD45RO
Long Name
CD45R, O isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
UniProt
Additional CD45RO Products
Product Documents for CD45RO Antibody (T200/797)
Product Specific Notices for CD45RO Antibody (T200/797)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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