CD45RO Antibody (UCHL-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33104

Novus Biologicals, part of Bio-Techne
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NBP2-33104
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Key Product Details

Species Reactivity

Human, Chimpanzee, Monkey

Applications

CyTOF-ready, Dual RNAscope ISH-IHC, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # UCHL-1

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD45RO Antibodies »

Product Specifications

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1)

Reactivity Notes

Common Marmoset

Localization

Cell surface

Specificity

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

182 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29631).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RO Antibody (UCHL-1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104]

Immunocytochemistry/ Immunofluorescence: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104]

Immunocytochemistry/Immunofluorescence: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104] - Immunofluorescence Analysis of human tonsil FFPE section stained with CD45RO antibody (UCHL-1)and goat anti-mouse HRP, detected using CF488A tyramide in Tyramide Amplification Buffer Plus.
Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104]

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104]

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104] - Staining of human tonsil
Flow Cytometry: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104]

Flow Cytometry: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104]

Flow Cytometry: CD45RO Antibody (UCHL-1) - Azide and BSA Free [NBP2-33104] - CD45RO expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD45RO antibody (0.5 ug/10^6 cells) or isotype control following surface staining protocol. PE-conjugated goat anti-mouse IgG, secondary antibody used to identify the positive staining on PPI negative lymphocyte gated population.
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Applications for CD45RO Antibody (UCHL-1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD45RO

CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.

Long Name

CD45R, O isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

Additional CD45RO Products

Product Documents for CD45RO Antibody (UCHL-1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD45RO Antibody (UCHL-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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