CD46 Antibody (122.2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33159

Clone 122.2 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-33159
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 122.2

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD46 Antibodies »

Product Summary for CD46 Antibody (122.2) - Azide and BSA Free

Immunogen

Recombinant human CD46 protein (Uniprot: P15529)

Localization

Cell surface

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-25222).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD46 Antibody (122.2) - Azide and BSA Free

Flow Cytometry: CD46 Antibody (122.2) - Azide and BSA Free [NBP2-33159]

Flow Cytometry: CD46 Antibody (122.2) - Azide and BSA Free [NBP2-33159]

Flow Cytometry: CD46 Antibody (122.2) - Azide and BSA Free [NBP2-33159] - FCM staining of human PBMCs using CD46 Antibody (122.2).

Applications for CD46 Antibody (122.2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD46

CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in regulation of complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells.

Alternate Names

CD46, MCP

Gene Symbol

CD46

Additional CD46 Products

Product Documents for CD46 Antibody (122.2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD46 Antibody (122.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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