Key Product Details

Species Reactivity

Mouse

Applications

Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Recombinant Monoclonal Rat IgG1 Clone # 974222

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD47 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived mouse CD47
Gln19-Pro158
Accession # NP_034711

Specificity

Detects mouse CD47 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for CD47 Antibody (974222) [PE]

CD47 Antibody (974222) [PE]

CD47 Antibody (974222) [PE] [FAB18661P] -

CD47 Antibody (974222) [PE] [FAB18661P] - Vial of PE conjugated antibody. PE has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 565 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 578 nm.

Applications for CD47 Antibody (974222) [PE]

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD47

CD47 antigen, also known as integrin associated protein (IAP), has a very broad tissue distribution. CD47 is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N glycosylated cell membrane glycoprotein of apparent molecular weight 47-52 kDa. It may play a role as a signaltransducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells.

Alternate Names

CD47, IAP, MER6, OA3

Additional CD47 Products

Product Documents for CD47 Antibody (974222) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD47 Antibody (974222) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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