CD47 Antibody (CD47/6362R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07224
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224]](https://resources.bio-techne.com/images/products/CD47-Antibody-CD47-6362R-Immunohistochemistry-Paraffin-NBP3-07224-img0002.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # CD47/6362R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary) (Uniprot: Q08722)
Localization
Cell Surface
Specificity
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08218)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for CD47 Antibody (CD47/6362R)
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224]
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Recombinant Rabbit Monoclonal Antibody (CD47/6362R).![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224]](https://resources.bio-techne.com/images/products/CD47-Antibody-CD47-6362R-Immunohistochemistry-Paraffin-NBP3-07224-img0001.jpg "Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224]")
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224]
Immunohistochemistry-Paraffin: CD47 Antibody (CD47/6362R) [NBP3-07224] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Recombinant Rabbit Monoclonal Antibody (CD47/6362R). Inset: PBS used instead of primary as secondary antibody negative controlApplications for CD47 Antibody (CD47/6362R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD47
Additional CD47 Products
Product Documents for CD47 Antibody (CD47/6362R)
Product Specific Notices for CD47 Antibody (CD47/6362R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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