CD5 Antibody (CD5/54/F6) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44920
![Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - IHC-Prediluted [NBP2-44920]](https://resources.bio-techne.com/images/products/CD5-Antibody-CD5-54-F6-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44920-img0001.jpg "Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - IHC-Prediluted [NBP2-44920]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine, Bovine, Chicken, Equine, Guinea Pig, Monkey, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CD5/54/F6
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment (around aa450-495) of human CD5 protein (intracellular region). (Uniprot: P06127 )
Localization
Cell surface
Specificity
Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
Marker
Mantle Cell Lymphoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for CD5 Antibody (CD5/54/F6) - IHC-Prediluted
Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - IHC-Prediluted [NBP2-44920]
Immunohistochemistry-Paraffin: CD5 Antibody (CD5/54/F6) - IHC-Prediluted [NBP2-44920] - Formalin-fixed, paraffin-embedded human tonsil stained with CD5 MAb (CD5/54/F6)Applications for CD5 Antibody (CD5/54/F6) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD5
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for CD5 Antibody (CD5/54/F6) - IHC-Prediluted
Product Specific Notices for CD5 Antibody (CD5/54/F6) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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