CD5 Antibody (OTI7A7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70360
![Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70360-img0003.jpg "Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
![Western Blot: CD5 Antibody (OTI7A7)Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Western-Blot-NBP2-70360-img0001.jpg "Western Blot: CD5 Antibody (OTI7A7)Azide and BSA Free [NBP2-70360]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Imaging Mass Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI7A7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD5 Antibody (OTI7A7) - Azide and BSA Free
Western Blot: CD5 Antibody (OTI7A7)Azide and BSA Free [NBP2-70360]
Western Blot: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.![Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70360-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]
Immunocytochemistry/Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5.![Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70360-img0008.jpg "Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]
Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Human Kidney tissue using anti-CD5 mouse monoclonal antibody.![Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70360-img0005.jpg "Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
![Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70360-img0006.jpg "Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
![Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70360-img0007.jpg "Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
![Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70360-img0002.jpg "Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
![Imaging Mass Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]](https://resources.bio-techne.com/images/products/CD5-Antibody-OTI7A7-Azide-and-BSA-Free-Imaging-Mass-Cytometry-NBP2-70360-img0009.jpg "Imaging Mass Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]")
Applications for CD5 Antibody (OTI7A7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Imaging Mass Cytometry
Validated from a verified customer review
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Western Blot
1:2000
Reviewed Applications
Read 1 review rated 4 using NBP2-70360 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD5
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for CD5 Antibody (OTI7A7) - Azide and BSA Free
Product Specific Notices for CD5 Antibody (OTI7A7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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