CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52645

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

CyTOF-ready, Dual RNAscope ISH-IHC, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # Campath-1H

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD52 Antibodies »

Product Summary for CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

Immunogen

Human lymphocytes.

Specificity

Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

Immunohistochemistry: CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free [NBP2-52645]

Immunohistochemistry: CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free [NBP2-52645]

Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-52645] - Staining of human thyroid cancer using anti-CD52 antibody. Formalin fixed human thyroid cancer slices were were stained at 5 ug/ml.
Dual RNAscope ISH-IHC: CD52 Antibody (Campath-1H) [NBP2-52645] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD52 mRNA (ACD RNAScope probe, catalog # 519381; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using rabbit monoclonal (Novus catalog # NBP2-52645) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC003) and green HRP chromogen (ACD Catalog # 322500).
Flow Cytometry: CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free [NBP2-52645]

Flow Cytometry: CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free [NBP2-52645]

Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-52645] - Flow-cytometry using anti-CD52 antibody Campath-1H. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Applications for CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD52

Human CD52 is a glycosylphosphatidylinositol (GPI)anchored antigen expressed in a number of different tissues.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP, biosimilar antibody, biosimilars

Gene Symbol

CD52

Additional CD52 Products

Product Documents for CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD52 Antibody (Campath-1H) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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