CD53 Antibody (104)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90952
Recombinant Monoclonal Antibody
![Western Blot: CD53 Antibody (104) [NBP2-90952]](https://resources.bio-techne.com/images/products/CD53-Antibody-104-Western-Blot-NBP2-90952-img0004.jpg "Western Blot: CD53 Antibody (104) [NBP2-90952]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Cynomolgus Monkey
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 104
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD53 Antibody (104)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus CD53 (Uniprot#: G7NW46; Glu107-Asn181).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CD53 Antibody (104)
Western Blot: CD53 Antibody (104) [NBP2-90952]
Western Blot: CD53 Antibody (104) [NBP2-90952] - Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.![Immunoprecipitation: CD53 Antibody (104) [NBP2-90952]](https://resources.bio-techne.com/images/products/CD53-Antibody-104-Immunoprecipitation-NBP2-90952-img0003.jpg "Immunoprecipitation: CD53 Antibody (104) [NBP2-90952]")
Immunoprecipitation: CD53 Antibody (104) [NBP2-90952]
Immunoprecipitation: CD53 Antibody (104) [NBP2-90952] - CD53 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. Lane B:0.5 mg Raji Whole Cell Lysate. 2 uL anti-CD53 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-cyno CD53 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted and observed band size: 34 kDa.Applications for CD53 Antibody (104)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD53
Alternate Names
CD53, MOX44, TSPAN25
Gene Symbol
CD53
Additional CD53 Products
Product Documents for CD53 Antibody (104)
Product Specific Notices for CD53 Antibody (104)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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