CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12059

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-12059AF350

Alexa Fluor 405

NBP3-12059AF405

Alexa Fluor 488

NBP3-12059AF488

Alexa Fluor 532

NBP3-12059AF532

Alexa Fluor 594

NBP3-12059AF594

Alexa Fluor 647

NBP3-12059AF647

Alexa Fluor 700

NBP3-12059AF700

Alexa Fluor 750

NBP3-12059AF750

Allophycocyanin

NBP3-12059APC

Biotin

NBP3-12059B

CoraFluor 1

NBP3-12059CL1

DyLight 350

NBP3-12059UV

DyLight 405

NBP3-12059V

DyLight 488

NBP3-12059G

DyLight 550

NBP3-12059R

DyLight 594

NBP3-12059DL594

DyLight 650

NBP3-12059C

DyLight 680

NBP3-12059FR

DyLight 755

NBP3-12059IR

FITC

NBP3-12059F

HRP

NBP3-12059H

Janelia Fluor 525

NBP3-12059JF525

Janelia Fluor 549

NBP3-12059JF549

Janelia Fluor 585

NBP3-12059JF585

Janelia Fluor 635

NBP3-12059JF635

Janelia Fluor 646

NBP3-12059JF646

Janelia Fluor 669

NBP3-12059JF669

mFluor Violet 450 SE

NBP3-12059MFV450

mFluor Violet 500 SE

NBP3-12059MFV500

mFluor Violet 610 SE

NBP3-12059MFV610

PE

NBP3-12059PE

PerCP

NBP3-12059PCP

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # HD77

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD53 Antibodies »

Product Summary for CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

Immunogen

This antibody was raised by immunising mice with human leukemia cells.

Specificity

This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

Western Blot: CD53 Antibody (HD77)ChimericAzide and BSA Free [NBP3-12059]

Western Blot: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Human lymph node (A), human thymus (B) and human spleen (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HD77 (NBP3-12059) at 0.1 ug/ml, 0.3 ug/ml and 0.5 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: CD53 Antibody (HD77) - Chimeric - Azide and BSA Free [NBP3-12059]

Immunocytochemistry/Immunofluorescence: CD53 Antibody (HD77) - Chimeric [NBP3-12059] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version HD77 (NBP3-12059) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12059, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD53

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Alternate Names

CD53, MOX44, TSPAN25

Gene Symbol

CD53

Additional CD53 Products

Product Documents for CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for CD53 Antibody (HD77) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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