Key Product Details

Species Reactivity

Human

Applications

Validated:

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TS2/9

Format

Azide and BSA Free

Concentration

0.98 mg/ml

View all CD58/LFA-3 Antibodies »

Product Specifications

Immunogen

Human Leukocyte Function Antigen-3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free

Western Blot: CD58/LFA-3 Antibody (TS2/9)Azide and BSA Free [NBP2-22542]

Western Blot: CD58/LFA-3 Antibody (TS2/9)Azide and BSA Free [NBP2-22542]

Western Blot: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/ Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]

Immunocytochemistry/ Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]

Immunocytochemistry/Immunofluorescence: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]

Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free [NBP2-22542]

Flow Cytometry: CD58/LFA-3 Antibody (TS2/9) [NBP2-22542] - Analysis of CD58 in BAF-3 cells compared to an isotype control (blue).
View 2 more images

Applications for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.98 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD58/LFA-3

LFA3 is a type 1 transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on most hematopoietic cells and on nonhematopoietic cells such as fibroblasts, endothelial and epithelial cells. Its expression is particularly high on memory T-cells and dendritic cells. LFA3 expression on antigen-presenting cells and target cells enhances T-cells antigen recognition through binding to its ligand, LFA2/CD2, on T-cells.

Alternate Names

CD58, LFA-3, LFA3

Entrez Gene IDs

965 (Human)

Gene Symbol

CD58

UniProt

P19256

Additional CD58/LFA-3 Products

Product Documents for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD58/LFA-3 Antibody (TS2/9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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