CD6 Antibody (3F7B5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34537
Clone 3F7B5 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]](https://resources.bio-techne.com/images/products/CD6-Antibody-3F7B5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34537-img0004.jpg "Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]")
![Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]](https://resources.bio-techne.com/images/products/CD6-Antibody-3F7B5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34537-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3F7B5
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for CD6 Antibody (3F7B5) - Azide and BSA Free
Immunogen
Human rheumatoid synovial T cell line ST-1
Localization
Cell surface and cytoplasmic
Marker
Negative Marker of T-regulatory Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32942).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD6 Antibody (3F7B5) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]
Immunocytochemistry/Immunofluorescence: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]](https://resources.bio-techne.com/images/products/CD6-Antibody-3F7B5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34537-img0003.jpg "Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]")
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD6 MAb (3F7B5).![Flow Cytometry: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]](https://resources.bio-techne.com/images/products/CD6-Antibody-3F7B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34537-img0002.jpg "Flow Cytometry: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]")
Flow Cytometry: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537]
Flow Cytometry: CD6 Antibody (3F7B5) - Azide and BSA Free [NBP2-34537] - Flow Cytometric Analysis of MOLT-4 cells. CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).Applications for CD6 Antibody (3F7B5) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD6
Alternate Names
CD6
Gene Symbol
CD6
Additional CD6 Products
Product Documents for CD6 Antibody (3F7B5) - Azide and BSA Free
Product Specific Notices for CD6 Antibody (3F7B5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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