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CD6 Antibody (C6/2884R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07555

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
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NBP3-07555-100ug
NBP3-07555-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # C6/2884R

Concentration

0.2 mg/ml

Product Summary for CD6 Antibody (C6/2884R)

Immunogen

Human recombinant full-length CD6 protein (Uniprot: P30203)

Localization

Cell Surface and Cytoplasmic

Marker

Negative Marker of T-regulatory Cells

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08281)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD6 Antibody (C6/2884R)

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (C6/2884R) [NBP3-07555]

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (C6/2884R) [NBP3-07555]

Immunocytochemistry/Immunofluorescence: CD6 Antibody (C6/2884R) [NBP3-07555] - Immunofluorescence staining of MOLT-4 cells using CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (green). Nuclei are stained with RedDot.
Immunohistochemistry-Paraffin: CD6 Antibody (C6/2884R) [NBP3-07555]

Immunohistochemistry-Paraffin: CD6 Antibody (C6/2884R) [NBP3-07555]

Immunohistochemistry-Paraffin: CD6 Antibody (C6/2884R) [NBP3-07555] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R).
Flow Cytometry: CD6 Antibody (C6/2884R) [NBP3-07555]

Flow Cytometry: CD6 Antibody (C6/2884R) [NBP3-07555]

Flow Cytometry: CD6 Antibody (C6/2884R) [NBP3-07555] - Flow Cytometric Analysis of MOLT-4 cells. CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).

Applications for CD6 Antibody (C6/2884R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD6

CD6 is a 100-130 kD single chain transmembrane glycoprotein also known as T12. It is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6, interacting with its ligand CD166 (also known as ALCAM), is involved in T cell development and activation, as well as thymocyte adhesion.

Alternate Names

CD6

Gene Symbol

CD6

UniProt

Additional CD6 Products

Product Documents for CD6 Antibody (C6/2884R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD6 Antibody (C6/2884R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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