Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C6/372

Concentration

0.2 mg/ml

View all CD6 Antibodies »

Product Specifications

Immunogen

Human recombinant CD6 protein (Uniprot: P30203)

Localization

Cell surface and Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34584)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CD6 Antibody (C6/372)

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (C6/372) [NBP2-32827]

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (C6/372) [NBP2-32827]

Immunocytochemistry/Immunofluorescence: CD6 Antibody (C6/372) [NBP2-32827] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (C6/372) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Immunohistochemistry-Paraffin: CD6 Antibody (C6/372) [NBP2-32827]

Immunohistochemistry-Paraffin: CD6 Antibody (C6/372) [NBP2-32827]

Immunohistochemistry-Paraffin: CD6 Antibody (C6/372) [NBP2-32827] - Formalin-fixed paraffin-embedded human tonsil stained with CD6 Monoclonal Antibody (C6/372)

Applications for CD6 Antibody (C6/372)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD6

CD6 is a 100-130 kD single chain transmembrane glycoprotein also known as T12. It is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6, interacting with its ligand CD166 (also known as ALCAM), is involved in T cell development and activation, as well as thymocyte adhesion.

Alternate Names

CD6

Gene Symbol

CD6

UniProt

P30203 (Human)

Additional CD6 Products

Product Documents for CD6 Antibody (C6/372)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD6 Antibody (C6/372)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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