CD6 Antibody (SPM547)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32828
![Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) [NBP2-32828]](https://resources.bio-techne.com/images/products/CD6-Antibody-SPM547-Immunocytochemistry-Immunofluorescence-NBP2-32828-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) [NBP2-32828]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM547
Concentration
0.2 mg/ml
Product Summary for CD6 Antibody (SPM547)
Immunogen
Human recombinant CD6 protein (Uniprot: P30203)
Localization
Cell surface and Cytoplasmic
Marker
Negative Marker of T-regulatory Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34789)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD6 Antibody (SPM547)
Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) [NBP2-32828]
Immunocytochemistry/Immunofluorescence: CD6 Antibody (SPM547) [NBP2-32828] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (SPM547) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) [NBP2-32828]](https://resources.bio-techne.com/images/products/CD6-Antibody-SPM547-Immunohistochemistry-Paraffin-NBP2-32828-img0001.jpg "Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) [NBP2-32828]")
Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) [NBP2-32828]
Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) [NBP2-32828] - Formalin-fixed paraffin-embedded human tonsil stained with CD6 Monoclonal antibody (SPM547).Applications for CD6 Antibody (SPM547)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD6
Additional CD6 Products
Product Documents for CD6 Antibody (SPM547)
Product Specific Notices for CD6 Antibody (SPM547)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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