CD69 Antibody (8B6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51607

Novus Biologicals, part of Bio-Techne
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NBP1-51607
NBP1-51607-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Imaging Mass Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8B6

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CD69 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD69 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD69 Antibody (8B6) - BSA Free

Western Blot: CD69 Antibody (8B6)BSA Free [NBP1-51607]

Western Blot: CD69 Antibody (8B6)BSA Free [NBP1-51607]

Western Blot: CD69 Antibody (8B6) [NBP1-51607] - Analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.
Immunohistochemistry-Paraffin: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Immunohistochemistry-Paraffin: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Immunohistochemistry-Paraffin: CD69 Antibody (8B6) [NBP1-51607] - Human tonsil tissue stained with CD69 antibody NBP1-51607. IHC-P image submitted by a verified customer review.
Flow Cytometry: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Flow Cytometry: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Flow Cytometry: CD69 Antibody (8B6) [NBP1-51607] - Analysis of Jurkat cells using CD69 mouse mAb (green) and negative control (purple).
View 2 more images

Applications for CD69 Antibody (8B6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
CD69 antibody validated for IHC-P and Imaging Mass Cytometry from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-51607 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD69

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3 and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection. The H1.2F3 antibody has been reported to augment T cell activation.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Gene Symbol

CD69

Additional CD69 Products

Product Documents for CD69 Antibody (8B6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD69 Antibody (8B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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