CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81050
Recombinant Monoclonal Antibody. Clone 3A1E was used by HLDA to establish CD designation.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 3A1E
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
CD7
Specificity
Recognises human CD7.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free
Western Blot: CD7 Antibody (3A1E)ChimericAzide and BSA Free [NBP2-81050]
Western Blot: CD7 Antibody (3A1E) [NBP2-81050] - Western Blot using anti-CD7 antibody (3A1E) [NBP2-81050]. Jurkat cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 3A1E [NBP2-81050] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa, but this protein is glycosylated at several residues. NBP2-81050 successfully detected CD7 in Jurkat cell lysate.Immunocytochemistry/ Immunofluorescence: CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free [NBP2-81050]
Immunocytochemistry/Immunofluorescence: CD7 Antibody (3A1E) [NBP2-81050] - Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody (3A1E) [NBP2-81050]. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3A1E [NBP2-81050] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81050, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.Applications for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD7
Alternate Names
CD7, LEU-9, TP41
Gene Symbol
CD7
Additional CD7 Products
Product Documents for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free
Product Specific Notices for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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