Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CD7 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 190 to 240 of human CD7 Molecule using the numbering given in entry NP_006128.1 (GeneID 924).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD7 Antibody

Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for IP at 6 ug per reaction. For blotting immunoprecipitated CD7, NBP2-32097 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: CD7 Antibody [NBP2-32097]

Immunohistochemistry: CD7 Antibody [NBP2-32097]

Immunohistochemistry: CD7 Antibody [NBP2-32097] - Sample: FFPE section of human stomach cancer infiltrating lymphocytes. Antibody: Affinity purified rabbit anti- CD7 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CD7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

UniProt

NP_006128.1 (Human)

Additional CD7 Products

Product Documents for CD7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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