Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CD7 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 190 to 240 of human CD7 Molecule using the numbering given in entry NP_006128.1 (GeneID 924).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD7 Antibody - BSA Free

Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for IP at 6 ug per reaction. For blotting immunoprecipitated CD7, NBP2-32097 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: CD7 Antibody [NBP2-32097]

Immunohistochemistry: CD7 Antibody [NBP2-32097]

Immunohistochemistry: CD7 Antibody [NBP2-32097] - Sample: FFPE section of human stomach cancer infiltrating lymphocytes. Antibody: Affinity purified rabbit anti- CD7 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097]

Western Blot: CD7 Antibody [NBP2-32097] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody NBP2-32097 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CD7 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

UniProt

NP_006128.1 (Human)

Additional CD7 Products

Product Documents for CD7 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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