CD74 Antibody (LN-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29465

Clone LN-2 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-29465
NBP2-29465-20ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Baboon, Rat (Negative)

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LN-2

Concentration

0.2 mg/ml

View all CD74 Antibodies »

Product Specifications

Immunogen

SU-DHL-4 lymphoma cells

Reactivity Notes

Does not react with Rat.

Localization

Cell surface and paranuclear globular

Specificity

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33088)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD74 Antibody (LN-2)

Western Blot: CD74 Antibody (LN-2) [NBP2-29465]

Western Blot: CD74 Antibody (LN-2) [NBP2-29465]

Western Blot: CD74 Antibody (LN-2) [NBP2-29465] - Western blot analysis of Raji cell lysate using CD74 antibody (LN-2).
Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465]

Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465]

Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) [NBP2-29465] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465]

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) [NBP2-29465] - Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Antibody (LN-2).
View 2 more images

Applications for CD74 Antibody (LN-2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunohistochemistry

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunofluorescence: (Methanol fixed): 1-4ug/ml.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD74

CD74, a Type-II transmembrane protein, is a unique member of RIP-processed protein family. It consists of an extracytoplasmic domain, a transmembrane segment and an intracytoplasmic domain. It is expressed in B-cells, monocytes, macrophages and other Class II MHC expressing cells. CD74 associates with alpha and beta chains of HLA-DR (MHC class II) and directs intracellular sorting of MHC class II molecules. It has a prominent role in antigen presentation and as an accessory signaling molecule. Cell-surface CD74 binds to MIF and its expression is required for MIF-mediated ERK1/2-phosphorylation, PGE2 production and cell proliferation. CD74 regulates B-cell differentiation and acts as a survival receptor through activation of NF-kB p65/RelA homodimer. Pathological role of CD74 has been identified in different cancers including Multiple Myeloma.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Gene Symbol

CD74

UniProt

P04233 (Human)

Additional CD74 Products

Product Documents for CD74 Antibody (LN-2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD74 Antibody (LN-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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