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CD79B Antibody (B29/123) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08356

Clone B29/123 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # B29/123

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for CD79B Antibody (B29/123) - Azide and BSA Free

Immunogen

Synthetic peptide representing the c-terminus from residue 215 of the murine CD79B polypeptide. (Uniprot: P40259)

Localization

Cell surface

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07438).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD79B Antibody (B29/123) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD79B Antibody (B29/123) - Azide and BSA Free [NBP3-08356]

Immunohistochemistry-Paraffin: CD79B Antibody (B29/123) - Azide and BSA Free [NBP3-08356]

Immunohistochemistry-Paraffin: CD79B Antibody (B29/123) - Azide and BSA Free [NBP3-08356] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79B Mouse Monoclonal Antibody (B29/123).
Immunohistochemistry-Paraffin: CD79B Antibody (B29/123) - Azide and BSA Free [NBP3-08356]

Immunohistochemistry-Paraffin: CD79B Antibody (B29/123) - Azide and BSA Free [NBP3-08356]

Immunohistochemistry-Paraffin: CD79B Antibody (B29/123) - Azide and BSA Free [NBP3-08356] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79B Mouse Monoclonal Antibody (B29/123).

Applications for CD79B Antibody (B29/123) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD79B

Murine CD79 is a 23/21 kDa disulfide-linked heterodimer composed of an alpha chain (CD79alpha/mb-1) and a beta chain (CD79b/B29) that associates non-covalently with membrane immunoglobulin (Ig) to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the pro-B cell stage prior to productive Ig gene rearrangements and remaining through all stages of B-cell differentiation prior to plasma cells. It has been proposed that the CD79 complex on pro-B cell surfaces may function to induce early B-cell differentiation. (1)

Alternate Names

B29, CD79B, IGB

Gene Symbol

CD79B

Additional CD79B Products

Product Documents for CD79B Antibody (B29/123) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD79B Antibody (B29/123) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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