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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # H35-17.2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen for this antibody was CD8.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for CD8 beta Antibody (H35-17.2) [FITC]

Flow Cytometry: CD8 beta Antibody (H35-17.2) [FITC] [NBP1-44038] - Staining of BALB/c splenocytes with Anti-Mouse CD8a APC and 0.125 ug of Rat IgG2b k Isotype Control FITC (left) or 0.125 ug of Anti-Mouse CD8b FITC (right). Cells in the lymphocyte gate were used for analysis

Applications for CD8 beta Antibody (H35-17.2) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
This H35-17.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD8 beta

The H35-17.2 monoclonal antibody reacts with the mouse CD8beta molecule. The CD8beta chain associates with the CD8alpha chain to form the CD8alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alphabeta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Alternate Names

CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37

Entrez Gene IDs

12526 (Mouse)

Gene Symbol

CD8B1

UniProt

Additional CD8 beta Products

Product Documents for CD8 beta Antibody (H35-17.2) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD8 beta Antibody (H35-17.2) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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