CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12080
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free [NBP3-12080]](https://resources.bio-techne.com/images/products/CD81-Antibody-1-3-3-22-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12080-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free [NBP3-12080]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 1.3.3.22
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma.
Specificity
1.3.3.22 antibody recognizes CD81. Human CD81 is a member of the tetraspanin family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. It is present on a wide variety of leukocytes.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free [NBP3-12080]
Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD81
Alternate Names
CD81, TAPA-1, TSPAN28
Gene Symbol
CD81
Additional CD81 Products
Product Documents for CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free
Product Specific Notices for CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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