CD81 Antibody (1D6) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81096

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-81096-0.025mg
NBP2-81096-0.2mg
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Key Product Details

Species Reactivity

Validated:

Human, Chimpanzee, Goat, Sheep

Applications

ELISA, Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 1D6

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD81 Antibodies »

Product Summary for CD81 Antibody (1D6) - Chimeric - Azide and BSA Free

Immunogen

This antibody was produced by immunizing mice with 5A6-aggregated OCI-LY8 B cells.

Specificity

This antibody is specific for human CD81, which is expressed on most tissues with the exception of red blood cells and platelets.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD81 Antibody (1D6) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1D6) - Chimeric - Azide and BSA Free [NBP2-81096]

Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1D6) - Chimeric - Azide and BSA Free [NBP2-81096]

Immunocytochemistry/Immunofluorescence: CD81 Antibody (1D6) [NBP2-81096] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81096, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.

Applications for CD81 Antibody (1D6) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody is a strong inducer of homotypic adhesion in hematolymphoid cells (Levy, 1998). This antibody also has an anti-proliferative effect, and is able to induce growth inhibition of B cell lines. Triggering of CD81 by this antibody induces a significant upregulation of the constituitive release of TNF-alpha by JY cells, an Eptsein-Barr virus-positive B lymphoblastoid cell line (Altomonte, 1995).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD81

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (1D6) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD81 Antibody (1D6) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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