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CD81 Antibody (M38) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44859

Novus Biologicals, part of Bio-Techne
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NBP1-44859
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline, Rabbit

Cited:

Feline

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # M38

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MOLT-4, human T-ALL cell line (NM_004356.3).

Reactivity Notes

Cat (100%). Use in Mouse reported in scientific publication( PMID: 32573489).

Specificity

The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Applications for CD81 Antibody (M38) [FITC]

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD81

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells R11; e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (M38) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD81 Antibody (M38) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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