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CD82/Kai-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81627

Novus Biologicals, part of Bio-Techne
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NBP1-81627
NBP1-81627-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CD82/Kai-1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NMGKLKQEMGGIVTELIRDYNSSREDSLQDAWDYVQAQVKCCGWVSFYNWTDNAELMNRPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD82/Kai-1 Antibody

Western Blot: CD82/Kai-1 Antibody [NBP1-81627]

Western Blot: CD82/Kai-1 Antibody [NBP1-81627]

Western Blot: CD82/Kai-1 Antibody [NBP1-81627] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-81627]

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-81627]

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-81627] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-81627]

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-81627]

Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody [NBP1-81627] - Immunohistochemistry analysis in human skin and skeletal muscle tissue. Corresponding RNA-seq data are presented for the same tissues.

Applications for CD82/Kai-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD82/Kai-1

Kangai 1 is a leukocyte surface glycoprotein with metastasis/tumor suppressor activity. It inhibits the progression of prostate, breast, lung, bladder, pancreatic and hepatocellular carcinomas. Lower levels of Kangai 1 gene expression correlate with the malignant development of cancers.

Alternate Names

CD82, IA4, KAI1, SAR2, ST6, TSPAN27

Gene Symbol

CD82

Additional CD82/Kai-1 Products

Product Documents for CD82/Kai-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD82/Kai-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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