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CD84/SLAMF5 Antibody (CD84.1.21) [PE/Cy5.5]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65929PECY55

Novus Biologicals, part of Bio-Techne
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NB100-65929PECY55
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # CD84.1.21

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse pre-B cell line 300.19 transfected with CD84 cDNA

Localization

Type I membrane protein

Specificity

NB100-65929 recognizes the human CD84 cell surface antigen, a highly glycosylated cell surface antigen of 64-82kD. CD84 is predominantly expressed by B lymphocytes, monocytes and macrophages, although lower levels of expression have also been reported on T cells, granulocytes and platelets.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD84/SLAMF5 Antibody (CD84.1.21) [PE/Cy5.5]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD84/SLAMF5

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Long Name

SLAM Family Member 5

Alternate Names

CD84, Ly-9B, SLAMF5

Gene Symbol

CD84

Additional CD84/SLAMF5 Products

Product Documents for CD84/SLAMF5 Antibody (CD84.1.21) [PE/Cy5.5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD84/SLAMF5 Antibody (CD84.1.21) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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