CD9 Antibody (10N2L6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15593

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15593-100ul
NBP3-15593-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10N2L6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD9 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 129-228 of human CD9 (P21926). YNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMIFGMIFSMILCCAIRRNREMV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD9 Antibody (10N2L6)

Western Blot: CD9 Antibody (10N2L6) [NBP3-15593]

Western Blot: CD9 Antibody (10N2L6) [NBP3-15593]

Western Blot: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of extracts of various cell lines, using CD9 antibody (NBP3-15593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593]

Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593]

Immunohistochemistry-Paraffin: CD9 Antibody (10N2L6) [NBP3-15593] - Human lung cancer using CD9 Rabbit mAb (NBP3-15593) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593]

Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593]

Immunoprecipitation: CD9 Antibody (10N2L6) [NBP3-15593] - Analysis of 300ug extracts of THP-1 cells using 3ug CD9 antibody (NBP3-15593). Western blot was performed from the immunoprecipitate using CD9 antibody (NBP3-15593) at a dilition of 1:1000.

Applications for CD9 Antibody (10N2L6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD9

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behavior in peripheral nerve.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (10N2L6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD9 Antibody (10N2L6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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