CD9 Antibody (IVA50) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-494

Novus Biologicals, part of Bio-Techne
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NB500-494
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Key Product Details

Species Reactivity

Validated:

Human, Bovine

Cited:

Human, Bovine

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # IVA50

Format

BSA Free

Concentration

1 mg/ml

View all CD9 Antibodies »

Product Specifications

Immunogen

Bovine thrombocytes.

Specificity

The antibody IVA50 reacts with CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD9 Antibody (IVA50) - BSA Free

Immunoprecipitation: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Immunoprecipitation: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Immunoprecipitation: CD9 Antibody (IVA50) [NB500-494] - Fig. 1. Immunoprecipitation of human CD9 from the biotin-labeled human platelets lysates. Lane 1: immunoprecipitation with anti-CD9 (IVA50) Lane 2: immunoprecipitation with anti-human CD9 control antibody Lane 3: immunoprecipitation with negative control.
CD9 Antibody (IVA50) - BSA Free Flow Cytometry: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Flow Cytometry: CD9 Antibody (IVA50) - BSA Free [NB500-494]

Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in Analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) purified antibody (concentration in sample 10 ug/ml) GAM APC.

Applications for CD9 Antibody (IVA50) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:50

Western Blot

1 ug/ml
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD9

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behaviour in peripheral nerve.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (IVA50) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD9 Antibody (IVA50) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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