CD90/Thy1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51963

Novus Biologicals, part of Bio-Techne
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NBP1-51963
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PEHTYRSRTNFTSKY, from the internal region of the protein sequence according to NP_006279.2.

Marker

Mesenchymal Cell Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CD90/Thy1 Antibody

Western Blot: CD90/Thy1 Antibody [NBP1-51963]

Western Blot: CD90/Thy1 Antibody [NBP1-51963]

Western Blot: CD90/Thy1 Antibody [NBP1-51963] - (0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963]

Immunohistochemistry-Paraffin: CD90/Thy1 Antibody [NBP1-51963] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CD90/Thy1 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

detection limit 1:64000

Western Blot

0.1-0.3 ug/ml
Application Notes
This antibody works in ELISA and Western Blot. Approx 26kDa band observed in Human Brain (Cerebellum) and Human Tonsil lysates (calculated MW of 17.9kDa according to NP_006279.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human)

Gene Symbol

THY1

UniProt

P04216

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD90/Thy1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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